Colistac

Description

• Colistac is a distinctive product which has antibacterial properties that enable it to bypass the multifactorial mechanisms of resistance of Clostridium perfringens which are otherwise effective against standard antibiotics, also it inhibits Gram-positive bacterial sortase due to its direct antibacterial activity toward toxigenic Clostridium perfringens
• Colistac can inhibit bacterial sortase which is an enzyme that is critically important for the life cycle of Gram-positive bacteria, especially Clostridium perfringens through combination to the hydrophobic side chains (the side chains of from the b6/b7 loop) through hydrophobic effect, embedded into the active site of sortase
• Colistac causes a decrease in pH that forces the bacterial cell of Clostridium perfringens to consume a great amount of energy to maintain the intracellular pH homeostasis of the cell resulting in elimination of Clostridium perfringens through prevention of its ability to grow, as Clostridium perfringens is a pH sensitive bacterium which cannot tolerate a pH less than 5.2 ± 1
• Colistac reduces the inflammation through the inactivation of NF-κB signaling pathway in intestinal epithelial cells challenged by Clostridium perfringens, as the main cell wall component of Clostridium perfringens is peptidoglycan (PGN) that is recognized by TLR2.2 or NOD1 in chicken intestinal epithelial cells, and thereby activating the NF-κB signaling pathway, and consequently the inflammatory responses were induced
• Colistac penetrates into the lipid assemblies causing perturbation of the lipid fraction of the plasma membrane of Clostridium perfringens, resulting in increasing the membrane permeability and in leakage of intracellular materials through its capacity to increase the permeability of phosphatidylcholine of unilamellar vesicles in the cell membrane, also it can cross the cell membrane, penetrating the interior of the cell and interacting with intracellular sites critical for activity of Clostridium perfringens
• Colistac plays an important role in the regulation of intestinal homeostasis due to having a strong anti-inflammatory effect which is effective in pathogen-infected chickens that leads to decrease of the up-regulation of pro-inflammatory factors induced by Clostridium perfringens
• Colistac has a distinctive ability to bind Clostridial toxins without reducing bioavailability of other nutrients in the body of poultry which reduces the negative impact of Clostridial toxins on performance and production of poultry from meat and eggs
• Colistac promotes restoration of the intestinal microflora by countering effects of intestinal damage caused by Clostridium perfringens through the inhibition of its overgrowth
• Colistac can significantly increase the ratio of villus height and crypt depth in duodenum and jejunum of chickens that contributes to intestinal regeneration and recovery of necrotic enteritis (NE) infected chickens